At the end of the course student shall be able to

At the end of the course student shall be able to

At the end of the course the student shall be able to acquire an integrated knowledge of organ, structure, function and its regulatory mechanism and apply the knowledge in understanding of disease and its treatment

Details of facilities to carry out theory and practical classes for UG students

Sno.	Faculty	Designation	Contact Details
1	Dr. Arghya Sur	Professor	drarghyasur@gmail.com
2	Dr. A. Vijaya Lakhshmi	Professor	vijayaakkupalli@gmail.com
3	Dr. Mitesh Sinha	Associate Professor	drmitesh@gmcraigarh.edu.in
4	Dr. Hemlata Gupta	Assistant Professorr	guptahemlata.gupta@gmail.com
5	Dr. Hari Mohan Prasad Sinha	Assistant Professorr	hmps2002@gmail.com
6	Manish Kumar Soni	Demonstration	manishkumarsoni750@mail.com

I) GOAL

The broad goal of the teaching of undergraduate students in physiologyaims at providing the student comprehensive knowledge of the normal functions of the organ systems of the body to facilitate an understanding of the physiological basis of health and diseases.

II) EDUCATIONAL OBJECTIVES:

1. At the end of the course, the student will be able to: describe the normalfunctions of all the organ systems, their regulatory mechanisms and interactions of the various systems for well-coordinated total body function.
2. Understand the relative contribution of each organ system in the maintenance of the milieu interior (homeostasis).

 

1. Explain the physiological aspects of normal growth and development. Analyze the physiological responses and adaptation to environmental Stresses.
2. Comprehend the physiological principles underlying pathogenesis and treatment of disease.
3. Correlate knowledge of physiology of human reproductive system in relation to National Family Welfare Program.

 

III) SKILL:

At the end of the course the student shall be able to:

1. Conduct experiments designed for study of physiological phenomena.
2. Interpret experimental/investigative data.
3. Distinguish between normal & abnormal data derived as a result of tests which he/she has performed and observed in the laboratory

 

IV) INTEGRATION:

At the end of the integrated teaching the student shall acquire an integrated knowledge of organ structure and function and its regulatory mechanisms
.

 

V) COURSE CONTENT:

Theory
List of topics.

 

1. GENERAL PHYSIOLOGY. (5 hours)

Must know.

- Introduction to Physiology - Branches of Physiology

- Functional organization of human body. - External and internal environment

- Homeostasis, Biofeedback mechanisms Cell Physiology:

-   Transport across cell membrane.

 

1. HEMATOLOGY : (15 hours)

            Must know

1. Composition of blood

1. Functions of blood

 

1. Plasma proteins: Types, concentration, functions.

1. Erythrocytes: Morphology, functions, normal count physiological variations in normal count & anaemia, polycythemia.

 

1. Haemopoesis: general concepts

1. Erythropoiesis: stages, Sites, regulation, reticulocyte & its clinical significance.

 

1. Haemoglobin: Functions, normal values, physiological variations.

1. Fate of erythrocytes: life span, Catabolism of Hb, bilirubin metabolism, jaundice.

 

1. Physiological basis of anaemia, nutritional anaemia.

1. Polycythemia: Primary & secondary.

Leukocytes: differences between R.B.C. & W.B.C., types of W.B.C.s normal count & differential W.B.C. count, physiological variations,

Properties, functions of W.B.C.s,

Granulopoiesis – stages, regulation,

Lymphopoiesis.

Pathological variations in total & differential W.B.C. count.

1. Immunity: definition, concept of antigen & antibody, types of immunity-Innate & Acquired, & their mechanism, cell mediated & humeral immunity, B lymphocytes, T lymphocytes & their types.

 

Primary & secondary response, basis of vaccination.

1. Blood groups: Landsteiner’s law,

 

ABO system – type A & B antigen, ABO system & inheritance, relation to transfusion, cross matching major & minor.

Rh System – inheritance, Rh incompatibility & blood transfusion, Erythroblastosisfoetalis.

1. Blood transfusion: indications, storage of blood & changes during storage, transfusion reactions.

 

1. Monocyte - macrophage system: Classification, functions, functions of spleen.

1. Hemostasis: definition, basic mechanisms of Hemostasis,

 

1. Platelets:structure, normal count & variations, functions, role in platelet plug formation, Hemostasis & clot retraction.

1. Blood coagulation: Coagulation factors in plasma, basic mechanism of

 

blood clotting, intrinsic & extrinsic pathways & difference between two pathways, role of calcium in coagulation, role of vitamin K, fate of clot. Anticoagulants – commonly used & their mechanism of actions, blood coagulation tests – bleeding time, clotting time.

Haemophilia.

- Body fluid compartments: role of water in body & its distributions, different body fluid compartments & composition of their fluid.

1. Blood volume: normal value, physiological & pathological variations, blood volume regulation in detail (To be taken at end of lectures on C.V.S, kidney and endocrines)

 

Desirable to know

1. Physical properties of blood.

 

1. Plasma proteins: Plasmapheresis, role of liver in plasma protein synthesis, relationship of diet & plasma protein synthesis.

1. R.B.C.: advantages of biconcave shape.

 

1. Bone marrow structure and cellular elements.

1. Common Haemoglobinopathies (Hbs, Hbc, Thalassaemia)

 

1. Method of determination of life span of R.B.Cs.

1. Types of jaundice.

 

1. Polycythemia – effects on haemodynamics,

1. Immunity: Antibody structure & types, antigen – antibody reactions.

 

1. Blood group: M. N. system, other blood groups.

1. Thrombocytosis, thrombocytopenia purpura.

 

1. Anticoagulants: used in vitro & in vivo.

1. Other blood coagulation tests.

 

1. Classification of hemorrhagic diseases, D.I.C.

1. Measurement of: total body water, blood volume, plasma volume, I.C.F. volume.

 

Nice to know

1. Blood component therapy.

 

1. Effects of splenectomy.

1. Plasmin system.

C) NERVE (5 hours)

Must know:

1. Distinctive histological features relevant to functions of nerve fibers.

 

1. Classification of nerve fibers: based on structure, diameter, functions and only for sensory nerves.

1. R.M.P. definition, production & maintenance, method of measurement, significance.

 

1. Action potential: definition,

Phases – depolarization, repolarization, ionic basis of depolarization &

Repolarization.

Production & propagation of A.P.,

Properties of A.P. and its significance.

1. Properties of nerve fibers.

 

1. Strength duration curve: chronaxie and factors affecting it.

1. Factors affecting conduction in a nerve.

 

Desirable to know:

1. Experimental techniques to study the mechanism of production of R.M.P.

 

1. A.P.: patch clamp, voltage clamp

1. Methods of recording of A.P.

 

D) MUSCLE (7 hours)

Must know.

1. Classification of muscles,

 

1. Structure of skeletal muscle:

Electron microscopic structure, muscle proteins – contractile, regulatory,

structural & enzymatic.

Sarcoplasmic tubular system:  concept  of  sarcoplasmic  triads  &  their

functions.

Neuromuscular transmission: Physiologic anatomy, events, N-M blocking & its clinical significance, applied aspect – myasthenia gravis

1. Excitation – contraction coupling.

 

1. Molecular basis of skeletal muscle contraction: sliding filament theory, power stroke – cross bridge cycle, role of calcium.

1. Energetics: fuel used by skeletal, muscle at rest & in exercise, metabolic pathways involved to yield A.T.P.,

 

Oxygen debt: definition, types (lactic, alactic), incurring of debt, repaying

the debt, significance.

1. Properties of skeletal muscle: excitability, refractory period (absolute, relative), conductivity, contractility – types (isometric, isotonic), effects of summations (multiple motor unit summation, frequency summation & tetanizibility), all or none law, extensibility & elasticity, fatiguability.

 

1. Factors affecting development of tension in the muscle:

1. number of motor units contracting- type of muscle, number of muscle fibers in each unit activated, supraspinal influences.

 

1. length – tension relationship

1. frequency of stimuli, duration of stimulation

 

1. load

1. type of contraction

 

1. Chemical composition of muscle fibers and ions.

1. E.M.G. (in brief)

 

1. Skeletal muscle circulation.

1. Smooth muscle: structure, distribution, types molecular mechanism of contraction, properties, regulation, and disorders.

 

Desirable to know

1. Heat liberated during various phases of contraction, Fenn effect.

 

1. Recording of muscle activity.

Nice to know

-     E.M.G. details.

E) RESPIRATORY PHYSIOLOGY (15 hours)

Must know:

1. Physiologic anatomy

 

1. Functions of respiratory system, non-respiratory functions of lung

1. Mechanics of respiration:

 

Ventilation:

Inspiratory & expiratory muscles, intraplural pressure, lung & thoracic compliance, factors affecting compliance, work of breathing, surface tension forces & role of surfactant, airway resistance, and elastic resistance.

1. Lung volumes and capacities. Measurement, physiological & significance (tidal volume, vital capacity, forced vital capacity – details)

 

1. Pulmonary ventilation, alveolar ventilation, alveolar dead space, - applied aspect,

Maximum breathing capacity & breathing reserve.

Diffusion of Gases:

-     Exchange of respiratory gases at alveolar – capillary membrane, factors

Affecting diffusion.

Gas Transport:

1. Transport of oxygen, role of Haemoglobin, oxygen dissociation curve & factors affecting it.

 

1. Transport of carbon dioxide

Control of Breathing:

Neural control – higher centers, reflexes.

Chemical control – central & peripheral chemoreceptors role of CO2, O2, H+
Pulmonary Circulation

1. Characteristics

 

1. Ventilation perfusion ratio

1. Respiratory adjustment in exercise.

 

Hypoxia: types & high altitude hypoxia

1. Artificial respiration:

1. Pulmonary function tests - principles

 

Desirable to know.

1. Method of determination of dead space, residual volume, functional residual capacity.

 

1. Oxygen therapy: indications, hazards of hyperbaric oxygen & use.

Nice to know

1. Concept of P50

 

1. Positive pressure breathing.

F) CARDIOVASCULAR PHYSIOLOGY (20 hours)

Must know:

1. Introduction, functions & importance of the system.

 

1. General organization.

1. Structure of heart, pericardium, myocardium, endocardium, nerve supply, Histology, details of cell junctions, syncytium, contractile & conducting fibers.

 

1. Properties of cardiac muscle: excitability, conductivity, contractility, Autorhythmicity, all or none law, long refractory period.

1. Junctional tissues of heart, pacemaker potential, action potential of cardiac muscle.

 

1. Generation & conduction of cardiac impulse.

1. ECG: lead arrangement, normal waves & their significance with reference to lead II

 

1. Cardiac cycle: pressure – volume changes, heart sounds & their clinical significance, correlation of pressure, volume, ECG, heart sounds in cardiac cycle.

1. Heart rate & its regulation.

 

1. Hemodynamics - def., blood flow, resistance

1. Cardiac output: normal values, physiological variations, factors affecting cardiac output – details, regulation, measurement – principles.

 

Blood pressure:

Normal levels, measurement, determinants, short term & long term

Regulation - details.

1. Capillary circulation, tissue fluid formation.

 

1. Lymphatic system: Anatomy & structure, formation of lymph, composition of lymph, functions of lymphatic system, lymph flow& factors affecting it.

1. Regional circulation: Physiologic anatomy, factors affecting, special features: coronary, cerebral , skin, portal

 

1. Adaptation of cardiopulmonary system to various grades of exercise.

1. Hemorrhagic shock – stages & compensatory mechanisms, effects on body, physiological basis of treatment in brief.

 

Desirable to know:

1. Ion channel & receptors (physiological, pharmacological & clinical significance)

 

1. E.C.G. – electrical axis of heart, heart blocks, arrhythmias, ischemia, infarctions.

1. Heart sounds: murmurs & their clinical significance.

 

Nice to know

1. Experimental methods of studying cardiovascular physiology,

 

1. Patho physiology of oedema

G) RENAL PHYSIOLOGY (10 hours)

Must know:

1. General introduction, structure & functions of kidney.

 

1. Renal circulation: special features from functional point of view.

1. Concept of clearance: to study renal physiology, for :

 

1. GFR – Inulin, Creatinine, basic principle of radioisotope method.

1. Renal blood flow – PAH

 

1. Concentration & dilution of urine – free water.

-     Formation of urine:

1. Glomerular stage – GFR (definition, dynamics, factors affecting & measurement))

 

1. Tubular stage – Reabsorption & secretion.

1. Sodium, potassium, glucose : details

 

1. Handling of water – concentration & dilution of urine.

1. Secretion of H+

 

1. Role of kidney in acid – base balance.

1. Physiology of micturition: basic reflex & control, cystometrogram.

 

1. Artificial kidney: basic principles of dialysis.

Desirable to know:

-     Experimental studies for renal functions.

 

Nice to know

- Disorders of micturition.

1. -     BODY TEMPERATURE REGULATION: (2 hours)

Must know:

- Homeothermia – Balance between heat gain & heat loss. - Regulation of body temperature,

Desirable to know:

-   Hyperthermia, Hypothermia.

1. ALIMENTARY SYSTEM: ( 12 hours)

 

Must know:

1. General introduction & organizational plan, innervations and blood supply.

 

Salivary secretion:

-  General principles & basic mechanisms of secretion composition, and

Functions of saliva, mechanism & regulation of salivary secretion.

Mastication and deglutition:

1. Three phases of deglutition- physiologic anatomy, mechanism & control Gastric secretion:

 

1. Functional  anatomy,  histology,  functions  of  stomach,  composition  of

Gastric juice, cellular mechanism of gastric secretion of acid, pepsin, intrinsic factor, other enzymes, phases of gastric secretion, regulation of gastric secretion.

-     Gastric Motility:

Electrical activity of stomach, pylorus, emptying of the stomach-pyloric pump, regulation & factors promoting & inhibiting emptying.

Pancreatic secretion:

1. Structure, composition & mechanism of secretion of electrolytes & enzymes, regulation of secretion.

 

1. Liver & gall bladder:

Microscopic structure, functions of liver, composition of bile, cellular mechanism of bile formation, enterohepatic circulation of bile salts, control of secretion, concentration & storage of bile in gall bladder. filling & evacuation of gall bladder functions of gall bladder

1. Intestinal secretion: Structure, innervations.

 

1. Composition & mechanism of secretion of small intestinal juice, regulation of secretion.

1. Secretion of large intestine: mucous, water, electrolyte.

 

1. Motility of small intestine:

Structure & innervation electrical activity of smooth muscle, resting membrane potential, slow waves, spike potentials, rhythmic segmenting contractions, peristalsis, control – neural & hormonal, functions of ileocecal valve.

1. Motility of large intestine:

 

1. Structure &innervation, mixing& mass movements, defecation reflex’ and

Its control

-     G.I. hormones: in brief.

Digestion & absorption:

Digestion & absorption of - carbohydrate,

1. Proteins

 

1. Fats

Absorption of water, electrolytes and vitamins.

Desirable to know:

1. Gastric mucosal barrier, experiments to study regulation of gastric juice secretion, disorders of secretion, peptic ulcer., inhibitors of gastric secretion

 

1. Effects of vagotomy, abnormal gastric motility vomiting.

1. Barium meal studies, endoscopy, biopsy.

 

1. Pathophysiology of small intestinal motility, paralytic ileus, diarrhea, obstruction.

1. Pathophysiology of colonic motility, irritable bowel syndrome, drugs, constipation.

 

1. Pancreatic function tests.

1. Gall stone, effects of removal of gall bladder

 

Nice to know

1. Disturbances of esophageal motility, spasm, achalasia, hiatus hernia.

 

1. Methods for study of intestinal absorption.

1. Effects of hepatectomy.

1. NUTRITION: (2 hours)

Must know:

-   Concept of balanced diet

-   Factors affecting caloric requirements

-   Requirements of various nutrients, sources, daily needs.

-   Nutrition under special conditions – pregnancy, lactation, growing child.

1. ENDOCRINE SYSTEM (10 hours)

 

Must know:

1. Introduction

 

1. Endocrine functions of Hypothalamus – releasing hormones, Mechanism of hormone action

1. Anterior pituitary hormones:

 

Functions, regulation, disorders.

Posterior pituitary hormones, ADH, Oxytocin. Functions, regulation, disorders.

Thyroid:

Hormone: synthesis, fate, functions, regulation, disorders.

-     Parathyroid:

Hormone: synthesis, functions, regulation, disorders – tetany.

1. Adrenal cortex: and medulla.

 

Hormone: secretion, functions, regulation, disorders

1. Pancreatic hormones:

 

Secretion, functions, regulation, disorders.

Desirable to know:

-     Radioimmunoassay.

Nice to know

-     Experimental studies.

L) REPRODUCTIVE PHYSIOLOGY: (8 hours)

Must know:

1. Sex chromosomes, sex determination, sex differentiation

 

1. Functional anatomy of reproductive system.

1. Puberty: changes in males & females and its control.

 

Spermatogenesis: stages & regulation
Semen analysis.

1. Testosterone: actions & regulation.

 

1. Male sexual act.

1. Menstrual cycle & ovarian cycle: Phases & hormonal regulation.

 

1. Menopause.

1. Ovulation: indicators & importance

 

1. Fertilization, implantation of ovum.

1. Functions of placenta

 

1. Physiology of pregnancy;

1. Maternal changes during pregnancy

 

1. Parturition: in brief – stages and mechanism.

1. Lactation: initiation & maintenance and control. Advantages of breast-feeding.

 

1. Contraception: to be taken as integrated topic.

Desirable to know:

1. Sex chromosomes: Barr bodies.

 

1. Development of genitals & gonads

Nice to know

-     Precocious & delayed puberty.

 

M) SPECIAL SENSES (8 hours)

Must know:

-     Eye:

Functional anatomy of eye, optics, microscopic structure of retina with retinal circuits, image formation,

Photochemistry of vision (photopic & scotopic vision, dark & light adoption),

Pupillary reflexes, Accommodation reaction, Errors of refraction and their correction, Colour vision – physiological & neural basis, accepted theory
of colour vision, classifications, basis of colour blindness and tests of colour blindness, significance.

Visual pathway – processing of information at different levels in visual pathway, organisation of visual cortex. Effects of lesion at different levels in visual pathway,

Movements of eyeballs: functions & control.

1. Ear:

 

Physics of sound, decibel system, Functions of external ear,

Functional anatomy of middle ear, functions of middle ear in detail, assessment of functions of middle ear, Functional anatomy of cochlea, functions of inner ear, place principle, theories of hearing.

1. Audiometry,

 

Auditory pathway & important features, auditory cortex (role in hearing & speech development)

-     Taste:

Functional anatomy of taste buds, different taste modalities, pathway, factors affecting taste sensation,

-     Smell:

Functional anatomy of receptors, primary olfactory sensations, pathway, factors affecting smell sensation,

Desirable to know:

1. Resolution of images,

 

1. Electrophysiology of internal ear: cochlear micro phonics.

Nice to know

1. Electrophysiology of retina.

 

1. Theories of hearing.

13) Speech –

Afferent and efferent mechanisms and role of cortical centers in speech, concept of cerebral dominance, development of speech, vocalization.

14) Memory:

Definition, stages, types, physiological basis, factors affecting, applied – amnesias in brief.

15) Learning:

Definition, types with examples, stages, factors influencing, role of motivation (positive & negative reinforcement, reward & punishment), physiological basis – role of different parts of CNS, structural, biochemical changes.

16) Conditioned reflexes:

Definition, difference between unconditioned & conditioned reflexes, development of conditioned reflexes, properties, significance.

17) Autonomic nervous system:

Organization and functions of Parasympathetic & Sympathetic and their control.

1. CSF :

 

Introduction, composition, normal CSF pressure, formation & circulation, functions, applied aspect – brief, blood brain barrier, blood CSF barrier.

19) “Physiology of Brain Death & changes after that” (This topic included vide Academic Council Resolution No. 303/2008 dated 29/07/2008)
Desirable to know:

 

General nervous system:

Neurotransmitters – details, susceptibility of synapse to hypoxia drugs etc., Mechanisms of referred pain, differences between superficial & deep pain, central analgesia system, supraspinal control of stretch reflex – details.

Thalamus - applied aspects – effects of lesions. Hypothalamus - applied aspects – effects of lesions Reticular formation – effects of lesion

EEG – Method of recording, abnormal patterns. Basal Ganglia – lesions, involuntary movements.

Cerebellum – Embryology, evolution, effects of stimulation & ablation. Cerebral cortex – effects of stimulation & ablation in different regions. Speech – aphasias.

 

Nice to know

Experimental studies – effects of stimulation & ablation.

Sleep, wakefulness – effects of sleep deprivation, disorders.

 

Books recommended:
1) Textbooks of Physiology :
Guyton	-	Textbook of Physiology
Ganong	-	Review of Medical Physiology
2) Reference Books :
Best and Taylor		-	Physiological basis of medical practice
Berne & levy.		-	Principles of Physiology
Dr. V.G. Ranade		-	Laboratory Manual and Journal of Physiology
			Practicals

Practicals Undergraduate
o Experimental Physiology:
1. Introducing use of various instruments in the laboratory
2. Types of circuits. Use of stimulator, pithing, smoking, varnishing
3. Muscle nerve preparation. Dissection and recording of Simple muscle twitch
4. Effects of Temperature
5. Effect of free and after load.
6. Effect of strength of stimulus
7. Study of two successive stimuli
8. Effect of frequent stimuli
9. Demonstration of phenomena of fatigue
10. Study of conduction velocity of nerve
11. Normal cardiogram and Effect of temperature
12. Study of properties of cardiac muscle
13. Effect of Stannius ligature I & II, heart-block (stannius ligatures).
14. Effect of simulation of vagus and crescent on cardiac contraction.
15. Study of effect of drug and identification of same
16. Perfusion of amphibian heart and
17. Effect of ions and drugs of physiological importance.
18. Decerebrate and spinal frog.

---

o  Hematology:
1. Total W.B.C. Count.
2. Differential W.B.C. count.
3. Total R.B.C. Count.
4. Hb Estimation.
5. P.C.V. & Blood indices.
6. E.S.R.
7. Bleeding time and clotting time.
8. Haemin crystals.
9. Study of microscope
10. Study of Peripheral smear
o Clinical Physiology
1. History taking and general examination.
2. Examination of cardiovascular system.
3. Measuring B.P. effect of posture, exercise, stress.
4. Examination of pulse.
5. Cardiac efficiency tests.
6. Examination of respiratory systems.
7. Spirometry.
8. Resuscitation: artificial respiration.
9. Closed thorax, cardiac massage.
10. Respiratory efficiency test
11. Examination of reflexes-superficial and deep.
12. Examination of motor systems
13. Examination of sensory system Perimetry.
14. Examination of cranial nerves.
15. Examination of alimentary system.
16. Examination of higher functions.
17. Visual acuity, Auditory tests.
18. Tests for sensation of taste and smell.
19. Recording of body temperature.

---

Demonstration:
Experimental Physiology:
1. Factors affecting muscular contraction, two successive stimuli, multiple stimuli, fatigue, temperature.
2. Velocity of nerve impulse.
3. Effect of drugs on heart - pilocarpine, acetylcholine, adrenaline, nicotine, and atropine.
4. Properties of cardiac muscle.
5. Perfusion of blood vessels.
Human Physiology:
6. Bone marrow smear.
7. Diameter of R.B.C., Price-Jones' Curve.
8. Reflex time and reaction time.
9. Stethography.
10. Phonocardiography and plethysmography.
11. Specific Gravity and platelet count.
12. Osmotic Fragility and reticulocyte count.
13. E.C.G.
14. Pregnancy tests.
15. Opthalmoscopy.
16. Ergography.

---

Mammalian Experiments.

17. Determination of blood volume in experimental animals.
18. Recording of movements of isolated loop of mammalian intestine and effect of drugs.
19. Mammalian blood pressure and respiration recording and factors influencing them.
Examination Pattern
UG
University Exam:
1. It is required to pass theory and practical exam separately. i.e. failing in any head means to reappear for both theory and practical exam after 2 months and then with the next batch
2. Total 200 Marks Exam. (140 Theories& 60 Practical)
3. Theory (Total 140)
a. Paper I (50 marks)
Cell physiology, General physiology, Biophysics, Physiology of Blood, Immunity, Cardiovascular physiology, excretoryphysiology, Respiratory physiology, Exercise physiology, underwater physiology, high altitude physiology.
b. Paper II (50 Marks)
Muscle and Nerve Physiology, Endocrine Physiology, Gastrointestinal physiology, Reproductive physiology, Autonomic nervous system, Special senses, Central nervous system physiology.
o INTERNAL 20 MARKS (derived from periodic & preliminary theory tests and periodic mcq tests)
Practical (Total 60)
Practical includes
o Hematology procedure
o Clinical procedure
o Instruments and graph/ photograph/charts
o Viva stations (include 2 stations of 10 marks each)
o INTERNAL 20 marks (to be derived from periodic practical exam and periodic viva voce)

List of Non teaching staff.

SNo.	Name	Designation
01.	Mr. Kaushal Prasad Sameer	Technicianst
02.	Miss. Panparmeshwari Dewdas	Store Keeper-cum-Clerk
03.	Miss Kaushlya Patre	Lab Technician
04.	Mr.Reshamlal Sidar	Laboratory Attendance
05.	Miss. Dhaneshwari Singh	Steno-typist
06.	Mr.Pintu Kumar Gupta	Any other category(Peon)