Department of Microbiology

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Goals and Objective of our Department

The Goal of the Department of Microbiology is to educate and train the medical students about the pathogens; their transmission, identification, treatment and prevention. And the laboratory services help in early diagnosis of the suspected infections among those who attend the concerned hospitals either as inpatient or outpatient departments. In the department of Microbiology we regularly expand the body of knowledge of this scientific field through research. The specific goals of the Department are to:

  1. Offer undergraduate students about the course of Microbiology within MBBS program as per MCI guidelines to enhance the intellectual foundation in microbiology and also prepare the students for to deal with life in a complex, dynamic technological world
  2. Offer undergraduate theory and practical’s of microbiology, following MCI guidelines in Toto. The department is involved in diagnostics microbiology and interdisciplinary research collaboration with other departments.
  3. prepare graduates and researchers with in-depth knowledge and research skills for professional careers in Microbiology
  4. enhance opportunities for research and scholarship for faculty members as well as undergraduate and graduate students
  5. preserve, add to, evaluate, and transmit knowledge in the field of Microbiology
  6. Serve society by promoting science in Govt. Medical College, Raigarh
Head of Departments message
Microbiology is rapidly growing branch of medicine that deals with the study of such minute creature that cannot seen through naked eyes. Microbes are many times Beneficial’s to us but not always and a specialized branch of Microbiology i.e., Medical Microbiology deals with those pathogenic microbe and establish their role in human health and diseases. It mainly focuses on the early diagnosis, treatment and prevention of the infectious diseases. There are four kinds of microbes that causing infections to the humans are: Bacteria, Virus, Fungi and Parasites.

A Medical Microbiologist studies the characteristics of pathogens, their modes of transmission, mechanisms of infection and growth. Utilizing this information, a treatment can be devised/modify. Medical microbiologists often serve as consultants for physicians, providing necessary information about the pathogens and suggesting treatment options. Other tasks may include the identification of potential health risks to the community as well as within the hospital or monitoring the evolution of potentially virulent or resistant strains of particular microbes, educating the community, medical and paramedical staff and assisting in the design of health practices.
Facilities in Department
Check day to day routine investigations requested by physicians/medical practitioners in hospital from inpatient and outpatient departments (Kirodimal Government Hospital). Check quality control both internal and external quality control as per guidelines
A) Handling Multidrug resistant pathogens.
B) Facility of Bio-safety cabinet level II to handle multidrug resistant pathogens.
C) Surveillance and management of Hospital associated infections.
D) Facility for Sero-diagnosis of various infections/diseases i.e., HIV, HAV, HBV, HCV, Dengue, Syphilis, and TORCH panel, etc.
E) Facility for isolation and identification of various bacterial, fungal and viral pathogens from the clinical samples
F) Facility of Culture and Sensitivity
G) Facility for various staining techniques, Gram’s, Zeihl-Neelsen, AFB, Fluorescent staining, dark ground Microscopy, etc.
H) Facility for automated blood culture system (BactiAlert)
I) Facility for Isolation and identification of Mycobacterium tuberculosis
J) Facility for identification and molecular characterization of infectious agents by RT-PCR
K) Service Laboratory is well acquainted with NABL guideline
L) Written SOPs for Laboratory investigations
Subject curriculum
COURSE CONTENT
III SEMESTER

1.GENERAL MICROBIOLOGY
A) Historical Introduction
B) Microscopy and Morphology of bacteria
C) Sterilization and Disinfection
D) Culture Media and Culture Methods
E) Identification of Bacteria
F) Bacterial Genetics

2.IMMUNOLOGY
A) Infection
B) Immunity
C) Antigens
D) Antibodies-Immunoglobulins
E) Antigen-Antibodies Reaction>
F) Complement System
G) Structure and Function of the Immune System
H) Immune Response
I) Hypersensitivity
J) Immunodeficiency Diseases
K) Autoimmunity
L) Immunology of Transplantation and Malignancy
M) Immunohematology

IV SEMESTER

SYSTEMIC BACTERIOLOGY
• Staphylococcus
• Streptococcus
• Streptococcus Pneumoniae
• Neisseria
• Corynebacterium
• Bacillus
• Anaerobic Bacteria I: Clostridium
• Anaerobic Bacteria II: Non- sporing Anaerobe
• Enterobacteraceae
• Vibrio
• Pseudomonas
• Yersinia, Pasteurella, Francisella
• Haemophilus
• Bordetella
• Mycobacterium: M. tuberculosis, NTM, M. leprae
• Spirochetes
• Mycoplasma
• Actinomycetes
• Rickettsiaceae and Chlamydiae

PARASITOLOGY
• General Introduction: Parasitology
• Protozoology- Amoeba
          Flagellates – 1 (Intestinal and Genital)
          Flagellates – 2 (Hemoflagellates)
          Sporozoa -1 (Malaria parasite and Babasia)
          Sporozoa -2(Opportunistic Coccidian Parasite)

• Helminthology –Cestodes
          Trematodes or Flukes
          Nematodes- 1 (Intestinal Nematodes)
          Nematodes- 2 (Nematodes that Rarely Infect Man)
          Nematodes- 3 (Somatic Nematodes)
• Medical Entomology

V SEMESTER

VIROLOGY
• General properties of Viruses
• Virus-Host Interaction
• Bacteriophages
• Poxviruses
• Herpesviruses
• Anenoviruses
• Picornaviruses
• Orthomyxoviruses
• Paramyxoviruses
• Arboviruses
• Rhabdoviruses
• Hepatitis Viruses
• Human Immunogenic Viruses: AIDS

MYCOLOGY
• General aspect
• Superficial and Subcutaneous Mycoses
• Systemic Mycoses
• Opportunistic Mycoses

CLINICAL MICROBIOLOGY
• Bloodstream Infections
• Respiratory Tract Infections
• Meningitis
• Urinary Tract Infections
• Sexually Transmitted Infections
• Diarrhea and Food Poisoning
• Skin and Soft Tissue Infections
• Healthcare-associated infections
• Pyrexia of Unknown Origin
• Zoonoses
Examination Pattern
• University Examination for 2nd Professional courses in Microbiology having 150 marks
• It includes Theory Paper I & Paper II marks, Practical marks, Pre-University marks, Internal assessment marks.
• Two Internal assessments Examination conducted whole academic session including Practical Examination and Viva voce.
• Pre-University Examination Conducted before University Examination in similar pattern.
• Also PCT examination conducted in every month end for improvement of students.
List of teaching and non teaching staff

Sl. No

Name

Designation

Phone Number

1

Dr. Patta Apparao

Prof. and HOD

2

Dr. Abhishek Gaur

Associate Professor

3

Mr. Prashant Panda

Assistant Professor

4

Dr. Pragya Khusro

Demonstrator

5

Mr. Sanad Patel

Technician


6

Mrs. Jyoti Nagdav

Technician


7

Mr. Nav Kumar Patel

Technician

8

Mr. Basant Kumar Patel

Technician

9

Mrs. Madhu Rajput

Technician

10

Mr. Praveen Partate

Technician

11

Mr. Suraj Kumar Malhotra

Lab Attendant

12

Kum. Sharda Sahu

Lab Attendant

13

Mr. Mukesh Kumar Dewangan

Store Keeper/Clerk

Awards
Prof. Patta Apparao
1- Member Indian Association of Medical Microbiology

Dr. Abhishek Gaur
1- Member, American Society for Microbiology (2010), Life Member (Membership Number: 56637523)
2- Member, International Society for Infectious Diseases (2011)
3- Member, Hospital Infection Society of India (2005), Life Member
4- Member, Indian Association of Medical Microbiologists (2005), Life member (U.P Chapter).
5- Associate Editor of Journal of AIDS and HIV Research
6- Member, Editorial Board of Asian Journal of Medical Sciences.
7- Editor in chief of International Journal of Microbial Research and Infections
Research paper(s) presented by Department faculty
  1. Bacteriological study of 125 strains of COAGULASE NEGATIVE STAPHYLOCOCCI; IAMM-Andhra Chapter 1995.
  2. 2. Serological study of STDs in truck drivers on highway in nellore district. IAMM-Andhra Chapter 1998.
  3. Abhishek Gaur; Invited Speaker: oral presentation;- “ESBL producing E. coli-Management and Treatment”; National Workshop on Laboratory based Surveillance of Antimicrobial Resistance and Application of WHONET in Laboratory Data Management; Manipal College of Medical Sciences, Pokhara, Nepal. 22th -27th September 2013.
  4. Abhishek Gaur; Invited Speaker: oral presentation;- “ESBL producing Enteric Bacteria-Management and Treatment”; National Workshop on Laboratory based Surveillance of Antimicrobial Resistance and Application of WHONET in Laboratory Data Management; Manipal College of Medical Sciences, Pokhara, Nepal. 12th -17th September 2011.
  5. Abhishek Gaur; Invited Speaker: oral presentation;- “Hypothesis”; National Workshop on Research Methodologies; Maitri College of Dentistry and Research Center, Anjora, DURG, Chattisgarh, India. 12th -13th April 2014.
  6. Abhishek Gaur, Bipin Chappagain, D R Bhatta, M T Ansari, H K Tiwari, J Mathuria, A N Ghosh, S Gokhale – “Bacterial colonization of mobile phones used by health care workers in western Nepal; a potential risk to nosocomial infections” Oral presentation of the XXXV annual conference of India Association of Medical Microbiologist, MICROCON 2011, Department of Microbiology, IMS, BHU, Varanasi. 24th -26th November 2011.
  7. M T Ansari, D R Bhatta, Abhishek Gaur, A N Ghosh, S Gokhale, J Mathuria, H K Tiwari- “Prevalence of vancomycin resistance and MDR in enterococci in western Nepal” Oral presentation of the XXXV annual conference of India Association of Medical Microbiologist, MICROCON 2011, Department of Microbiology, IMS, BHU, Varanasi. 24th -26th November 2011.
  8. Abhishek Gaur, T.M.Mohapatra, P. Prakash, A. Bhattachar’jee, Shampa Anupurba – “Clonal diversities and integron carriage in multidrug resistant nosocomial isolates of Acinetobacter baumannii” Poster presentation of the 47th Annual Inter-science Conference on Antimicrobial Agents and Chemotherapy (ICAAC), American Society for Microbiology (ASM), Chicago, USA. 17th -20th September 2007.
  9. Abhishek Gaur, T.M.Mohapatra, Shampa Anupurba– “Detection of Integrons in Nosocomial Isolates of Acinetobacter baumannii, found in north India”Oral presentation of the IX National Conference of HISI, Department of Microbiology, GMCH Chandigarh, India. 16th to 18th Feb 2007.
  10. T. M. Mohapatra, Abhishek Gaur, Shampa Anupurba– “Prevalence of Nosocomial infection in a Tertiary care hospital” IX National Conference of HISI, Department of Microbiology, GMCH. Chandigarh, India. 16th to 18th Feb 2007.
  11. Abhishek Gaur, Atul Garg, Pradyot Prakash, Shampa Anupurba, T. M. Mohapatra – “Carbapenem resistance, Metallo beta-lactamase and Integrons: Study in Nosocomial isolates of Acinetobacter baumannii”  Abstract of 15th Annual Conference of Indian association of Pathologist & Microbiologist (U. P. Chapter), Institute of Medical Sciences, BHU, Varanasi (U.P.), India. 11th November 2006.
  12. Abhishek Gaur, T.M.Mohapatra, Shampa Anupurba- “Antibiotic abuse: Carbapenem resistance in Nosocomial isolates of Acinetobacter baumannii”Abstract of the XXX National Conference of Indian Association of Medical Microbiologists, GMC, Nagpur, India. 26th to 29th Oct. 2006.
  13. Abhishek Gaur, Shampa Anupurba, T.M.Mohapatra– “Minimal Inhibitory concentration (MIC) pattern of Acinetobacter species to five commonly used drugs”Abstract of the VIII National Conference of Inficon, Apollo Hospital, Hyderabad, India. 28th to 30th January 2005.
  14. Abhishek Gaur, Shampa Anupurba, T.M.Mohapatra - Detection of integron in nosocomial strains of Acinetobacter species by integrase gene PCR”: An Indo-Nepal study, Abstract of First Annual Conference of U.P. Chapter- IAMM, Institute of Medical Sciences,  BHU, Varanasi, India. 3rd and 4th April 2005.
  15. Garg Atul, Anupurba S, Gaur Abhishek– “Pattern of clinical isolates from blood culture, a retrospective study”Abstract of 13th Annual Conference of Indian association of Pathologist & Microbiologist (U. P. Chapter) M.L.N. Medical College,  Allahabad (U.P.), India. 5th December 2004.
  16. Abhishek Gaur, Anupurba S, Mohapatra T.M. – “Acinetobacter species: A major isolates of nosocomial infections- Clinical significance & antimicrobial susceptibility”Abstract of the XXVII National Conference of Indian Association of Medical Microbiologists, Mumbai, India. 5th – 9th November 2003.
  17. Abhishek Gaur, H K Tiwari, Rahul Goyal, M R Sen– “A study on parasitic infection in Lumbini zone, Nepal”Abstract of the XXVII National Conference of Indian Association of Medical Microbiologists, Mumbai, India. 5th – 9th November 2003.
Research publication by Department faculty
  1. Prevalence of malaria from blood smear examination: an eleven-year retrospective study. original article,1st author. International journal 0f medical research professionals-ISSN-2454-6356
  2. A Demographic study of sputum smear positive cases of tuberculosis in Raigarh district- full length research article,1st author. Iinternational journal of current medical research-ISSN-2320-1339
  3. Seroprevalence of sexually transmitted diseases among pregnant women in a tertiary care hospital-original article,1st author. International journal of medical research professionals-ISSN-2454-6356
  4. Emerging Infections with rare Human Pathogen; Shewanella International; Indian Journal of public Health Research and Development.
  5. Study of opportunistic infections of intestinal parasitic and bacterial in HIV\AIDS POSITIVE individuals and correlation with CD4 count. Uunique journal of medical and dental sciences-ISSN-2347-5579.
  6. D Bhatta, L M Cavaco, G Nath, K kumar, Abhishek Gaur, S Gokhale and D R Bhatta. 2016-“Association of Ponton Valentine leukocidin (PVL) gene in methicillin resistant Staphylococcus aureus (MRSA)”: a matter of concern for community infections. BMC infectious disease; 16(199):1-6.
  7. Apparao P, Pattanayak MK, Abhishek Gaur. 2015- “A Demographic study of Sputum Smear Positive Cases of Tuberculosis in Raigarh District. Int. J of Current Med. Res.; 5(1):403-05.
  8. D Bhatta, L M Cavaco, G Nath, Abhishek Gaur, S Gokhale and D R Bhatta. 2015-“Threat of multi-drug resistant Staphylococcus aureus” in western Nepal: a hospital based prospective study. Asian Pacific Journal of Tropical Dis; 5(8): 617-21.
  9. D Bhatta, S Gokhale, A L Sharma, U Gupta, Abhishek Gaur, S Gowda, S Raut, S Thapa and R Khadka. 2014-“ Carrier state of Hemophilus influenze type b (Hib), Streptococcus pneumoniae, Streptococcus pyogenes, Neisseria meningitides, and Corynebacterium diptheriae among school children in Pokhara, Nepal”. Asian Pacific Journal of Tropical Dis; 4(1): 45-49.
  10. D Bhatta, Abhishek Gaur, Supram HS. 2013 –“Bacteriological profile of blood strem infections among febrile patients attending a tertiary care center of western Nepal”. Asian J. Med. Sciences; 4(3): 92-98.
  11. Ghosh AN, Bhatta DR, Ansari MT, Tiwari HK, Mathuria JP, Gaur A, Supram HS and Gokhale S. May 2013- “Application of WHONET in the Antimicrobial resistance surveillance of Uropathogens: A first user experience from Nepal. J. of Clin and Diagnostic Res; 7(5): 845-48.
  12. D Bhatta, S Gokhale, MT Ansari, HK Tiwari, Abhishek Gaur, J Mathuria& AN Ghosh. 2012 – “Gonococcal infections: The trends of antimicrobial susceptibility of Neisseria gonorrhoeae in Western Nepal”. Nepal J. of Med. Siences;1(2):74-8.
  13. D Bhatta, S Gokhale, MT Ansari, HK Tiwari, Abhishek Gaur, J Mathuria& AN Ghosh. 2011. Stethoscopes: A possible mode for transmission of nosocomial pathogens. Journal of Clinical & Diagnostic Research; November (Suppl-1): 5(6); 1173-76.
  14. Amitabh Bhattacharjee, Malay Ranjan Sen, Pradyot Prakash, Shampa Anupurba, Abhishek Gaur, Gopal Nath. 2010 - “Observation on integron carriage among clinical isolates of klebsiella pneumoniae producing” ESBLs. Ind. J. Med. Microbiol;28(3):207-10.
  15. J. Dinesh Kumar, Yogesh K. Negi, Abhishek Gaur and Deepshikha Khanna. 2009 - “Detection of virulence genes in Staphylococcus aureus isolated from paper currencies”. Int. J of Infect. Dis. ; 13: e450-e455.
  16. A. Bhattacharjee, M R Sen, P. Prakash, Abhishek Gaur & S. Anupurba. 2008 - “Increased Prevalence of ESBL producers in Neonatal Septicemic Cases at a Tertiary Referral Hospital”. Ind. J. Med. Microbiol; 26(4):356-60.
  17. H K Tiwari, Darshan Sapkota, Abhishek Gaur, J Mathuria, Asim Singh & M R Sen. 2008 - “Molecular typing of clinical Staphylococcus aureus isolates from northern India using coagulase gene PCR- RFLP”. Southeast Asian J Trop Med Public Health;39:467-73.
  18. A. Bhattacharjee, M. R. Sen, P. Prakash, Abhishek Gaur & S. Anupurba. 2008. “Prevalence of inducible AmpC-ß-lactamase producing Pseudomonas aeruginosa in a tertiary care hospital in Northern India”. Ind. J. Med. Microbiol;26(1): 89-90.
  19. Abhishek Gaur, A Garg, P Prakash, S Anupurba, and T M Mohapatra. 2008 – “Observation on Carbapenem resistance by MIC in nosocomial isolates of Acinetobacter species: an experience at a tertiary care hospital in North India” J. Health Popul Nutr;26(2):183-88.
  20. Abhishek Gaur, P Prakash, S Anupurba, T M Mohapatra. 2007 – “Possible role of Integrase gene Polymerase Chain Reaction as an epidemiological marker: Study on multi-drug resistant Acinetobacter baumannii isolated from nosocomial infections”. Int. J. Antimicrob. Agents;Apr,29(4): 446-50.
  21. Abhishek Gaur, A Bhattacharjee, S Anupurba, P Prakash, M R Sen and T M Mohapatra. 2007 - “Clinical significance and Epidemiology of multi drug resistant Acinetobacter species isolated from nosocomial infection”: An experience of a tertiary care hospital in North India. Ind. J. Prev. & Soc. Med;38(1,2): 7-11.
  22. Apparao P, Pattanayak MK, Abhishek Gaur. 2015- “A Demographic study of Sputum Smear Positive Cases of Tuberculosis in Raigarh District. Int. J of Current Med. Res.; 5(1):403-05.
  23. Jyoti Aswani, Ramesh Murthy, Sweta Sao, Mihir Pattanayak. 2015-‘ Modified Hodge Test for detection of carbapenemase in clinical isolates of Pesudomonas aeruginosa. IJSRM; 3(8): 3485-88.
  24. Jyoti Aswani, Mihir pattanayak, Sweta Sao. 2015-“ Incidence of Carbapenem resistant Pesudomonas aeruginosa isolated from patient with lower respiratory tract infection in ICU. IJSRM; 3(7): 3289-92.

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