1. Describe the molecular and functional organization of a cell and list its subcellular components :
2. Delineate structure, function and inter-relationships of biomolecules and consequences of deviation from normal
3. Summarize the fundamental aspects of enzymology and clinical application wherein regulation of enzymatic activity is altered
4. Describe digestion and assimilation of nutrients and consequences of malnutrition
5. Integrate the various aspects of metabolism and their regulatory pathways
6. Explain the biochemical basis of inherited disorders with their associated sequelae
7. Describe mechanism involved in maintenance of body fluid and pH homeostasis
8. Outline the molecular mechanisms of gene expression and regulation, the principles of genetic engineering and their application in medicine
9. Summarize the molecular concepts of body defence and their application in medicine
10. Outline the biochemical basis of environmental health hazards, biochemical basis of cancer and carcinogenesis
11. Familiarize with the principles of various conventional and specialized laboratory investigations and instrumentation analysis and interpretation of a given data
12. The ability to suggest experiments to support theoretical concepts and clinical diagnosis

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The Department of Biochemistry has been developed with the elaborate infrastructure as prescribed by the Medical Council of India.

There is one separate Demonstration Rooms with sitting capacity of 50 students. It has a facility of Black Board, OHP and LCD projector.

64 students at a time can perform practical work. Each seat is provided with separate glassware’s and laboratory tools. The laboratory is well equipped with all the facilities for biochemical investigations in blood, urine, CSF and gastric juice. Both qualitative and quantitative experiments are being carried out by UG students. There is a separate preparation room attached with UG laboratory.

There is a separate well equipped PG laboratory for post-graduates and Research students. Faculty members also carry out their Research Projects in PG Laboratory. PG Research lab is equipped with Spectro photometer, Electrophoresis, TLC, Homogenizer, Vaccum Pump, Flam Photometer etc.

The Clinical Biochemistry Lab is situated at Kirodimal Govt. Hospital Raigarh C.G. which is attached with Late Shri Lakhiram Agrawal Memorial Medical College Raigarh. It is a well equipped Clinical Biochemistry Laboratory which caters to the need of patients of 300 bedded teaching hospital & rendering 24 hours biochemical investigative services. Routine biochemical investigations including Diabetic profile, liver function test, Kidney function test, Lipid profile, diagnostic enzymes and other specialized tests (Hb Electrophoresis) are being carried out on the request of clinicians for better diagnosis and management. Laboratory has both internal and external quality control.

Department of Biochemistry has started Sickle cell unit in August 2016 with collaboration of SCIC Raipur & running successfully. The sickle cell unit provide screening, Diagnosis, treatment of patients suffering from sickle cell anemia of Raigarh region.

There is a Departmental Library-cum-Seminar room with sitting capacity of 25 students at a time and has good collection of books (113). Departmental seminars are being conducted regularly where latest researches in Biochemistry are being discussed.

All the faculty members have their own rooms with Wi-Fi internet connections.

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Theory

There is one separate Demonstration Rooms with sitting capacity of 50 students. It has a facility of Black Board, OHP and LCD projector.

64 students at a time can perform practical work. Each seat is provided with separate glassware’s and laboratory tools. The laboratory is well equipped with all the facilities for biochemical investigations in blood, urine, CSF and gastric juice. Both qualitative and quantitative experiments are being carried out by UG students. There is a separate preparation room attached with UG laboratory.

There is a separate well equipped PG laboratory for post-graduates and Research students. Faculty members also carry out their Research Projects in PG Laboratory. PG Research lab is equipped with Spectro photometer, Electrophoresis, TLC, Homogenizer, Vaccum Pump, Flam Photometer etc.

The Clinical Biochemistry Lab is situated at Kirodimal Govt. Hospital Raigarh C.G. which is attached with Late Shri Lakhiram Agrawal Memorial Medical College Raigarh. It is a well equipped Clinical Biochemistry Laboratory which caters to the need of patients of 300 bedded teaching hospital & rendering 24 hours biochemical investigative services. Routine biochemical investigations including Diabetic profile, liver function test, Kidney function test, Lipid profile, diagnostic enzymes and other specialized tests (Hb Electrophoresis) are being carried out on the request of clinicians for better diagnosis and management. Laboratory has both internal and external quality control.

Department of Biochemistry has started Sickle cell unit in August 2016 with collaboration of SCIC Raipur & running successfully. The sickle cell unit provide screening, Diagnosis, treatment of patients suffering from sickle cell anemia of Raigarh region.

There is a Departmental Library-cum-Seminar room with sitting capacity of 25 students at a time and has good collection of books (113). Departmental seminars are being conducted regularly where latest researches in Biochemistry are being discussed.

Classification and general functions of lipids, nomenclature and classification of fatty acids, essential fatty acids, phospholipids, characterisation of lipids (saponification number, iodine number), rancidity of fat, derived lipids (cholesterol, bile salts, steroid hormones).

All the faculty members have their Balanced diet, SDA of food components, BMR, BMI, PEMs, total parenteral nutrition.

Fat soluble vitamins (A,D,E,K) water soluble vitamins (vitamin c, thiamine, riboflavin, niacin, pantothenic acid, biotin, pyridoxine, folic acid, one carbon metabolism, cobalamine).

Calcium, iron, phosphorus, magnesium, sulfur, zine, selenium, maganese, Cobalt, coppoer, iodine, sodium, potassium, chloride, fluoride.

Overview and regulation of metabolic pathways, methods of study of metabolic pathways, metabolic profile in different organs (brain, skeletal muscle, cardiac muscle, adipose tissue, liver).

Digestion and absorption of carbohydrates, fate of glucose, glycolyisis, BPG shunt, PDH, TCA cycle, Glycogen metabolism and glycogen storage disorders, Gluconeogenesis, HMP shunt, Cori's cycle, Uronic acid pathway Galactose metabolism fructose metabolism, regulation of blood sugar level, glucose tolerance test, biochemical insights to diabetes, starvation and obesity, mucopolysaccharidoses, metabolism of alcohol

Laws of thermodynamics, general concept of oxidation and reduction, oxidases, conenzymes involved in redox reaction, cytochromes, oxygenases, high energy compounds, malate aspartate shuttle, glycerolphosphate shuttle, electron transport chain, chemiosmotic theory of ATP synthesis, uncouplers and inhibitors of ETC.

Digestion and absorption of proteins, general metabolism of Aminoacids (Transamination, deamination, transport of aminoacids), urea cycle. Intracellular protein degradation, ubiquitin and proteasome pathways, metabolism of individual aminoacids (glycine, serine, methionine, cystein, phenylalanine, tyrosine, tryptophan, branched chain aminoacids, acidic aminoacids, basic aminoacids, sulfur containing aminoacids) inborn errors of aminoacids metabolism.

Digestion and absorption of lipids, transport of lipids and lipoproteins, oxidation of fatty acids, de novo synthesis of fatty acids, ketone body metabolism, metabolism of adipose tissue, fatty liver cholesterol- structure and metabolism, metabolism of bile acids and steroid hormones, synthesis of TAG and phosopholipids.

Structure of heme and hemogblobin, biosynthesis of heme, Porphyrias, Bilirubin metabolism and jaundice transport of gases by hemoglobin and oxygen dissociation curve, hemoglobin variants and related disorders

Purines, Pyrimidines, nucleosides, nucleotides, purine, boisynthesis (denovo and salvage pathway), degradation of purines, uric acid and gout, lesch-nyhan syndrome, pryimidine synthesis, degradartion of pyrimidines.

Structure and functions of nucleic acids, DNA organisation and chromosome structure, DNAreplication, Mutations and DNA repair mechanisms, inhibitors of replication, transcription, posttranscrpitional processing, structure and function of different types of RNAs, genetic code, translation and protein synthesis, post-translational modifications, inhibitors of transcription and translation, mitochondrial DNA and related disorders] protein trageting and sorting, Regulation of genetic expression, princples of heredity and genetic transmission of dieseases, chromosomal aberrations, Recombinant DNA Technology and Gene therapyRestriction endonuleases, vectors, hosts, cDNA and gene library, gene therapy, PCT, Blotting techniques, hybridoma technology, RFLP, human genome project.

Innate and acquired immunity, hypersensitivity reaction, structure and function of immunoglobulins, antigen-antibody reaction, complement system, HLA and MHCs.

Reactive oxygen species and free radicals, generation of free radicals, damage caused by free radicals, free radical scavenger system, antioxidants.

Cyt P450, phase one reactions, phase two reactions, oxidation, reduction, conjugation, hydrolysis.

Liver function tests, renal function test thyroid function test, gastric function tests, pancreatic function tests.

Radioactivity, types of radiations, half life, Measurement of radioactivity, radiation protection, applications of radioisotopes in diagnosis, therapy and research, RIA..

Chemical Stress, water pollutions, air pollution, heavy metal poisoning, toxic substances in food stuffs.

Signal transduction and mechanism of hormone action, biochemistry of cancer tumour markers, chromatography and electrophoresis, ELISA.

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Part-I : Qualitative test (Carbohydrates, protein, Urine analysis)

Part-II : Quantitative test (Diabetic profile, Lipid profile, RFT, LFT, CSF etc.)

Part-III: Laboratory instrumentation ( pH meter, colorimeter, electrophoresis, TLC, etc)

Part-IV : Case report (Clinical case discussion)

Teaching- learning Methodology

1. Didactic lectures: 3 hours/ week.

2. Tutorials : 1.30 hours / week .

3. Demonstration and Practical : 7.30 hours /week

Text/Reference Books Recommended

1. Medical Biochemistry Ed. DM Vasudevan, Sreekumari S & Kanan Vaidyanathan

2. Lippincotts Biochemistry Ed Harvey

3. Biochemistry Ed. Lubert Stryer. W.H. Freeman and Company, New York.

4. Principles of Biochemistry. Ed. Lehinger, Nelson and Cox. CBS Publishers and distributors.

5. Harper”s Biochemistry, Ed. R.K. Murray, D.K. Granner, P.A. Mayes and V.W. Rodwell. Appleton and Lange, Stamford, Connecticut

6. Tietz Textbook of Clinical Chemistry. Ed. Burtis and Ashwood. W.B. Saunders Company

Examination and marks distribution

Ist Terminal – 4th week of December – 1st week of January

Pre University – June – July

University (final) – July – august

Marks distribution

Theory			Practical	Internal assessment	Total
Paper I	Paper II	ORAL	40	40	200
50	50	20
120

** Internal assessment will be calculated on the basis of PCT, Class test, Seminar, 1st Terminal, and Pre university Examination.

Paper -1

Cell and subcellular Organelles, Acid Base Balance and pH, Body fluids, Water And Electrolyte Balance, Enzymes, Chemistry of Carbohydrates, Chemistry of Proteins, Chemistry of Lipids, Nutrition, Vitamins, Mineral Metabolism

Introduction To metabolism, Metabolism of Carbohydrates, Biological Oxidation, Metabolism of Aminoacids and Proteins, Metabolism of Lipids, Heme Synthesis and Breakdown, Chemistry And Metabolism of Nucleotides, Molecular Chemistry, Recombinant DNA Technology And Gene Therapy, Immunology, Free Radicals And Antioxidants, Detoxification And Xenobiotics, Organ Function Test, Radioisotopes in Medicine, Environmental Biochemistry, Advanced Biochemistry.

**syllabus of Paper I and Paper II subject to change year wise as per university guidelines.

List of Teaching and non teaching staff.

SNo.	Name	Designation	Contact Details
01.	Dr. Hemontika Chakraborty	Professor & HOD	hemontikachakraborty@yahoo.co.in
02.	Dr. Harish Kumar	Assistant Professor	harishuraon26@gmail.com
03.	Dr. Amit Raj Jangade	Demonstrator	amiraj5@gmail.com
04.	Dr. Vinita Banjare	Demonstrator	banjarevinita@gmail.com

List of Non teaching staff.

SNo.	Name	Designation	Contact Details
01.	Mr. Akshay Vartak	Biochemist	Aki230490@gmail.com
02.	Mr. Neeraj Dewangan	MLT	Nkd2569@gmail.com
03.	Mr. Naresh Dhruw	MLT	Nareshbdhruw120@gmail.com
04.	Mr. Khemchand	MLT	Kcmlt31km@gmail.com
05.	Mrs. Sarita Raj	MLT	Saritaraj9056@gmail.com
06.	Mr. Ramkumar Khairwar	MLT	mrkhairar@gmail.com
07	Mr. Arvind Bhagat	AG-II	arvindbhagat5556@gmail.com
08.	Mr. Lalit Dewangan	Lab Attendant	Lalitdewgan5694@gmail.com
09.	Mr.Pushpraj Tomar	Ward Boy	-
10.	Miss Bhanu Lahre	Chaukidar	Bhanulahrey13@gmail.com
11.	Mr. Satish Panda	Lab Attendant	-

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Department of Biochemistry successfully organised a National CME on "Biochemical aspects of inborn error of Metabolism" which was held on 17th Jan 2015. (2 credit hours awarded by CGMC ).

1st Position achieved by MBBS 1st Year student in MBBS state level quiz competition organized by 6th AMBI CHHATTISGARH STATE CHAPTER POINT OF CARE TESTING Dept. of Biochemistry CIMS Bilaspur C.G.

Research Paper

• "A STUDY ON ROLE OF CA 15.3 IN THE BREAST CANCER PATINTS OF CHHATTISGARH"
• 
  
• Published by – Dr. Harish Kumar Uraon. Published in – International journal of scientific research volume 7 issue 7 August 2018.
• Evaluation of oxidative stress in rheumatoid arthritis. Rajesh Kumar Shrivastava, Vikas Gupta, Manish Kumar, Nita Garg. Indian Journal of Applied Research accepted for publication in December 2016 issue.

• 1. Evaluation of inflammatory markers in Essential hypertension. Rajesh Kumar Shrivastava, Nita Garg. Indian Journal of Applied Research 2016;6(11):45- 46.
• 2. A prospective study to see the effect of fenugreek seed powder as hypoglycaemic agent       on type II Diabetes Mellitus”. Rajesh Kumar Shrivastava, Mukesh Kumar Meena, Meena Varma, Susmit Kosta &  Ravindra Kumar.International Journal of Bioassay 2013; 2(10):1344-1348.
• 3. Is TSH alone can be treated as diagnostic marker for Thyroid Disorders. Rajesh Kumar Shrivastava, Mukesh Kumar Meena, Meena Varma, Rajesh Kumar Jha, S. Kosta , Ravindra Kumar. Indian Journal of Applied Research 2013;3(11):361- 363.
• 4. First Phase Insulin secretion, Insulin Sensitivity, Ghrelin, Glp-1 and Pyy level changes 72 hours after Sleev Gastrectomy  in obese Diabetic Patients: The Gastric hypothesis. Mukesh Kumar Meena, M.K.S. Shaikh, Meena Varma, Rajesh Kumar Shrivastava  &  Jain V.K.International Journal of Bioassay 2013; 2(11):1448-1452
• 5. Comparative study of biochemical changes before & after 3 days of Bariatric Surgery in morbid obese patient. Mukesh Kumar Meena, M.K. Shaikh, Mohit Bhandari, Meena Varma, Rajesh Kumar  Shrivastava and Vivek Jain JARBS/2013/961(4).
• 6. Comparative study of tobacco chewer & smokers as a risk factor for cardiovascular diseas. Dr. Rajesh Kumar Shrivastava, , Rajesh Kumar Jha, & Ravindra Kuma Indian Journal of Applied Research  January 2014 issue 2014;4(01):26-27
• 7. Prevalence of overt and subclinical thyroid disease in patients tested for thyroid profile in CIMS Bilaspur (C.G.). Vikas Gupta, Prashant Nigam, SKB Patil. Int. J. of clinical biochemistry and research 2016:3(3):343-347
• 8. Status of some haematological and antioxidant parameters in SCA patients of Chhattishgarh region. Vikas K.Gupta, neelima Singh, S.W.Masram, SKB Patil. J. of med sci and res. Vol:03, issue 07, page 6886-6891: July 2015
• 9. Status of small Dense LDL in cardiovascular disease subjects taking drug therapy. Pranshi Mishra, Neelima Singh, Puneet Rastogi, Snajeev Singh, Vikas Gupta. Int J of health disease and research.Vikas Gupta. Vol.5: issue: 5 ; May 2015.
• 10. Lipid profile and Insulin resistance in Sub clinical hypothyroid subjects in Eastern U.P.  Region. Suchita Saxena, Neelima Singh, Vikas Gupta, SP Saxena .Int J of Contemporary medicine . Vol : 2, Issue : 2: 82- 87. Jan 2014
• 11. Status of antioxidants vitamin Plasma Malondialdehyde (MDA) and in sickle cell anemia patients of Chhattisgarh region.Vikas Gupta, Neelima Singh, Prashant Nigam,Jayant shah, SKB Patil.Int J of Contemporary medicine . Jan- Jun 2014; 2(1): 62-64 – 33.
• 12. Variation in serum electrolyte in sickle cell patients in Chhattishgarh Population.Vikas Gupta, Singh AK, saha J, Nigam P, Baburao SK, Singh N. IJSID, 2012; 2(1): 239- 243
• 13. Role of Immunoturbidimetric plasma fibrin D-dimer test in patients with coronary artery disease as well as ischemic heart disease in emergency medicine”. Ajay Singh, Vikas Gupta, Neelima singh, sanjeev K. Singh  Int J Cur Biomed Phar Res. 2011; 1(2): 31 – 33. Effect of Chemotherapy on Tumor Marker (Ca 15.3) and Serum in Breast Cancer Patients of Southern Eastern Region of Chhattisgarh; 6 Years Follow up Study. Priyanka Chandel,  Harish Uraon, Vivek Choudhary. International Journal of Clinical Biochemistry and Research ISSN No. 2394-6369 2016;3(1):16-22)
• 14. Evaluation of BRCA1 5382insc and serum ca15-3 levels in breast cancer patients from Chhattisgarh State. Onkar Prasad Kashyap, Harish Kumar Uraon, Pradeep Kumar Patra, Prafulla Kumar Khodiar, Vivek Chaudhry, LVKS Bhaskar, Ind. J. Med. Biochem. ISSN No. 0972-1207 Vol. 18, No. 1, 2014.
• 15. Effect of Hydroxyurea therapy on erythrocyte membrane Na+- K+ ATPase activity, Hbf and haematological indices in sickle cell disease patients Harish Kumar, Uraon, Onkar Prasad Kashyap, Pradeep Kumar Patra, Debapriya Rath, Hrishikesh Mishra, Sharja Phuljhele, Shashank Gupta, Prafulla Kumar Khodiar, Ind. J. Med. Biochem. ISSN No. 0972-1207 Vol. 17, No. 2, 2013.